mouse il-12 p70 antibody Search Results


room temperature with anti il 12p40  (Miltenyi Biotec)
90
Miltenyi Biotec room temperature with anti il 12p40
Room Temperature With Anti Il 12p40, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il-12+p70+antibody/pm30428349-614-4-9?v=Miltenyi+Biotec
Average 90 stars, based on 1 article reviews
room temperature with anti il 12p40 - by Bioz Stars, 2026-08
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biotinylated anti il 12p70  (R&D Systems)
92
R&D Systems biotinylated anti il 12p70
FIGURE 1. Inhibition of allergic diarrhea disease by nasal treatment with <t>IL-12p70</t> naked DNA plasmid. A, The incidence of allergic diarrhea was reduced in mice treated nasally with IL-12p70 DNA when compared with mice treated with empty vector plasmid. Allergic disease was induced in these mice by s.c. immunization and then repeated oral challenge (SC/ PO) with OVA. B, OVA-specific IgE Abs are reduced in the serum of allergic diarrhea-afflicted mice treated nasally with IL-12p70 DNA. C, To- tal IgE Abs are reduced in the serum of allergic diarrhea-afflicted mice treated with naked IL-12p70 DNA. The data are expressed as the mean SD and are representative of five independent experiments. Statistical dif- ferences between IL-12p70 DNA- and empty vector-treated mice (, p 0.01 and , p 0.05) are indicated.
Biotinylated Anti Il 12p70, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il-12+p70+antibody/pm15905591-78-19-24?v=R%26D+Systems
Average 92 stars, based on 1 article reviews
biotinylated anti il 12p70 - by Bioz Stars, 2026-08
92/100 stars
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mouse il 12 p70 antibody  (R&D Systems)
94
R&D Systems mouse il 12 p70 antibody
FIGURE 1. Inhibition of allergic diarrhea disease by nasal treatment with <t>IL-12p70</t> naked DNA plasmid. A, The incidence of allergic diarrhea was reduced in mice treated nasally with IL-12p70 DNA when compared with mice treated with empty vector plasmid. Allergic disease was induced in these mice by s.c. immunization and then repeated oral challenge (SC/ PO) with OVA. B, OVA-specific IgE Abs are reduced in the serum of allergic diarrhea-afflicted mice treated nasally with IL-12p70 DNA. C, To- tal IgE Abs are reduced in the serum of allergic diarrhea-afflicted mice treated with naked IL-12p70 DNA. The data are expressed as the mean SD and are representative of five independent experiments. Statistical dif- ferences between IL-12p70 DNA- and empty vector-treated mice (, p 0.01 and , p 0.05) are indicated.
Mouse Il 12 P70 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il-12+p70+antibody/pmc07045464-131-152-166?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
mouse il 12 p70 antibody - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
anti il 12 p40 p70  (Miltenyi Biotec)
94
Miltenyi Biotec anti il 12 p40 p70
FIGURE 1. Inhibition of allergic diarrhea disease by nasal treatment with <t>IL-12p70</t> naked DNA plasmid. A, The incidence of allergic diarrhea was reduced in mice treated nasally with IL-12p70 DNA when compared with mice treated with empty vector plasmid. Allergic disease was induced in these mice by s.c. immunization and then repeated oral challenge (SC/ PO) with OVA. B, OVA-specific IgE Abs are reduced in the serum of allergic diarrhea-afflicted mice treated nasally with IL-12p70 DNA. C, To- tal IgE Abs are reduced in the serum of allergic diarrhea-afflicted mice treated with naked IL-12p70 DNA. The data are expressed as the mean SD and are representative of five independent experiments. Statistical dif- ferences between IL-12p70 DNA- and empty vector-treated mice (, p 0.01 and , p 0.05) are indicated.
Anti Il 12 P40 P70, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il-12+p70+antibody/pm41618616-120-44-57?v=Miltenyi+Biotec
Average 94 stars, based on 1 article reviews
anti il 12 p40 p70 - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
rat monoclonal anti-mouse il-12 (p70) antibody  (Angio-Proteomie)
N/A
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FIGURE 1. Inhibition of allergic diarrhea disease by nasal treatment with IL-12p70 naked DNA plasmid. A, The incidence of allergic diarrhea was reduced in mice treated nasally with IL-12p70 DNA when compared with mice treated with empty vector plasmid. Allergic disease was induced in these mice by s.c. immunization and then repeated oral challenge (SC/ PO) with OVA. B, OVA-specific IgE Abs are reduced in the serum of allergic diarrhea-afflicted mice treated nasally with IL-12p70 DNA. C, To- tal IgE Abs are reduced in the serum of allergic diarrhea-afflicted mice treated with naked IL-12p70 DNA. The data are expressed as the mean SD and are representative of five independent experiments. Statistical dif- ferences between IL-12p70 DNA- and empty vector-treated mice (, p 0.01 and , p 0.05) are indicated.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Nasal IL-12p70 DNA prevents and treats intestinal allergic diarrhea.

doi: 10.4049/jimmunol.174.11.7423

Figure Lengend Snippet: FIGURE 1. Inhibition of allergic diarrhea disease by nasal treatment with IL-12p70 naked DNA plasmid. A, The incidence of allergic diarrhea was reduced in mice treated nasally with IL-12p70 DNA when compared with mice treated with empty vector plasmid. Allergic disease was induced in these mice by s.c. immunization and then repeated oral challenge (SC/ PO) with OVA. B, OVA-specific IgE Abs are reduced in the serum of allergic diarrhea-afflicted mice treated nasally with IL-12p70 DNA. C, To- tal IgE Abs are reduced in the serum of allergic diarrhea-afflicted mice treated with naked IL-12p70 DNA. The data are expressed as the mean SD and are representative of five independent experiments. Statistical dif- ferences between IL-12p70 DNA- and empty vector-treated mice (, p 0.01 and , p 0.05) are indicated.

Article Snippet: After electrophoresis, proteins were transferred to a polyvinylidene difluoride microporous membrane (Immobilon; Millipore) and the membrane was reacted with biotinylated anti-IL-12p70 (48110.11, rat IgG1; R&D Systems) and then incubated with a biotin-streptavidin complex (ABC-AP kit; Vector Laboratories).

Techniques: Inhibition, Plasmid Preparation

FIGURE 2. Reduction of IgA and IgG hyperresponsiveness in large in- testinal B cells and of Th2 cells responses by nasal administration of IL- 12p70 DNA. A, The frequency of OVA-specific IgA, IgG, IgG1, and IgM AFCs in the large intestine of mice treated with IL-12p70 DNA or empty vector plasmid. B, OVA-specific IL-4 and IFN- production were analyzed by intracellular staining using FACS analysis. In vivo treatment with IL- 12p70 DNA reduced the predominant Th2-type Ag-specific responses by large intestinal mononuclear cells isolated from diarrhea-afflicted mice. The mononuclear cells isolated from the large intestine (1.0 106 cells/ well) or spleen were cultured with OVA (1 mg/ml) for 4 days. After in- cubation, cells were harvested and subjected to intracellular staining with anti-IL-4 and IFN-. The data are expressed as the percentage of cytokine- positive cells in large intestinal CD4 T cells. The data are expressed as the mean SD and are representative of four independent experiments. Sta- tistical differences between IL-12p70 DNA- and empty vector-treated mice (, p 0.01) are indicated. N.D., Not detected.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Nasal IL-12p70 DNA prevents and treats intestinal allergic diarrhea.

doi: 10.4049/jimmunol.174.11.7423

Figure Lengend Snippet: FIGURE 2. Reduction of IgA and IgG hyperresponsiveness in large in- testinal B cells and of Th2 cells responses by nasal administration of IL- 12p70 DNA. A, The frequency of OVA-specific IgA, IgG, IgG1, and IgM AFCs in the large intestine of mice treated with IL-12p70 DNA or empty vector plasmid. B, OVA-specific IL-4 and IFN- production were analyzed by intracellular staining using FACS analysis. In vivo treatment with IL- 12p70 DNA reduced the predominant Th2-type Ag-specific responses by large intestinal mononuclear cells isolated from diarrhea-afflicted mice. The mononuclear cells isolated from the large intestine (1.0 106 cells/ well) or spleen were cultured with OVA (1 mg/ml) for 4 days. After in- cubation, cells were harvested and subjected to intracellular staining with anti-IL-4 and IFN-. The data are expressed as the percentage of cytokine- positive cells in large intestinal CD4 T cells. The data are expressed as the mean SD and are representative of four independent experiments. Sta- tistical differences between IL-12p70 DNA- and empty vector-treated mice (, p 0.01) are indicated. N.D., Not detected.

Article Snippet: After electrophoresis, proteins were transferred to a polyvinylidene difluoride microporous membrane (Immobilon; Millipore) and the membrane was reacted with biotinylated anti-IL-12p70 (48110.11, rat IgG1; R&D Systems) and then incubated with a biotin-streptavidin complex (ABC-AP kit; Vector Laboratories).

Techniques: Plasmid Preparation, Staining, In Vivo, Isolation, Cell Culture

FIGURE 5. Induction of IL-12p70 protein in the large intestine by nasal administration of IL-12p70 DNA. An- ti-IL-12p70 mAb used did not cross- react with the IL-12p40 molecule. A, IL-12p70-positive cells were detected near large intestinal lymphatic vessels after nasal IL-12p70 DNA treatment. B, The IL-12p70 protein was also de- tected in large intestinal extracts using immunoprecipitation and blotting analysis. Recombinant murine IL- 12p70 (rIL-12p70) protein (lane 1) was used as a positive control for the detection of IL-12p70 (B). C, IL- 12p70-producing cells were stained with fluorescence-conjugated anti- CD11c in the large intestine.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Nasal IL-12p70 DNA prevents and treats intestinal allergic diarrhea.

doi: 10.4049/jimmunol.174.11.7423

Figure Lengend Snippet: FIGURE 5. Induction of IL-12p70 protein in the large intestine by nasal administration of IL-12p70 DNA. An- ti-IL-12p70 mAb used did not cross- react with the IL-12p40 molecule. A, IL-12p70-positive cells were detected near large intestinal lymphatic vessels after nasal IL-12p70 DNA treatment. B, The IL-12p70 protein was also de- tected in large intestinal extracts using immunoprecipitation and blotting analysis. Recombinant murine IL- 12p70 (rIL-12p70) protein (lane 1) was used as a positive control for the detection of IL-12p70 (B). C, IL- 12p70-producing cells were stained with fluorescence-conjugated anti- CD11c in the large intestine.

Article Snippet: After electrophoresis, proteins were transferred to a polyvinylidene difluoride microporous membrane (Immobilon; Millipore) and the membrane was reacted with biotinylated anti-IL-12p70 (48110.11, rat IgG1; R&D Systems) and then incubated with a biotin-streptavidin complex (ABC-AP kit; Vector Laboratories).

Techniques: Immunoprecipitation, Recombinant, Positive Control, Staining

FIGURE 6. Nasal IL-12p70 DNA treatment cured allergic diarrhea. A, Frequency of allergic diarrhea de- creased after IL-12p70 DNA treat- ment (n 9 per group). B, The symp- toms of diarrhea were completely inhibited after three treatments with IL-12p70 DNA. C, Infiltration of eo- sinophils and basophils into the large intestine was blocked by the IL-12p70 DNA administration. The red arrows point to eosinophils and the blue ar- rows point to basophils. D, Results of the ELISPOT assay used to determine the OVA-specific Ig responses in the large intestinal mononuclear cells. The data are expressed as the mean SD and are representative of three in- dependent experiments. Statistical differences between IL-12p70 DNA- and empty vector-treated mice (, p 0.01) are indicated.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Nasal IL-12p70 DNA prevents and treats intestinal allergic diarrhea.

doi: 10.4049/jimmunol.174.11.7423

Figure Lengend Snippet: FIGURE 6. Nasal IL-12p70 DNA treatment cured allergic diarrhea. A, Frequency of allergic diarrhea de- creased after IL-12p70 DNA treat- ment (n 9 per group). B, The symp- toms of diarrhea were completely inhibited after three treatments with IL-12p70 DNA. C, Infiltration of eo- sinophils and basophils into the large intestine was blocked by the IL-12p70 DNA administration. The red arrows point to eosinophils and the blue ar- rows point to basophils. D, Results of the ELISPOT assay used to determine the OVA-specific Ig responses in the large intestinal mononuclear cells. The data are expressed as the mean SD and are representative of three in- dependent experiments. Statistical differences between IL-12p70 DNA- and empty vector-treated mice (, p 0.01) are indicated.

Article Snippet: After electrophoresis, proteins were transferred to a polyvinylidene difluoride microporous membrane (Immobilon; Millipore) and the membrane was reacted with biotinylated anti-IL-12p70 (48110.11, rat IgG1; R&D Systems) and then incubated with a biotin-streptavidin complex (ABC-AP kit; Vector Laboratories).

Techniques: Enzyme-linked Immunospot, Plasmid Preparation